Representative data for AKT/ERK Activation Multispecies InstantOne™ ELISA. Left: MCF7 cells were pre-treated with 10 µM wortmannin washed and unstimulated (-) or stimulated(+) with 10 ng/mL insulin for 10 min. Cells were analyzed for phosphorylated AKT 1/2/3 by InstantOne™ ELISA. Right: A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor) for 3 hours (-) or treated(+) with 10 ng/mL EGF for 10 min. Cells were analyzed for phosphorylated ERK 1/2 by InstantOne™ ELISA.